Differential migrants are species in which one population class migrates farther than another. White-throated sparrows (Zonotrichia albicollis), which migrate into the southern United States each autumn in large numbers, have been proposed as a candidate differential migrant (Cristol et al. 1999). Using Bird Banding Lab data, we tested for latitudinal variation in the sex ratio of White-throated Sparrows in the Atlantic and central flyways. We found that the proportion of females increases with decreasing latitude in at least the Atlantic flyway, strongly suggesting that females migrate farther than males. Sex ratios calculated from those banding data were compared to those of museum specimens collected at the same latitudes, as well as single-season population samples at three latitudes in the heart of the winter range. Broad agreement was found using the three independent methods of sampling the sex ratio, so we conclude that White-throated Sparrows are differential migrants.